IMARCS Foundation

Module 23

Biosecurity and Quarantine

What biosecurity means, what carries disease, working one system at a time, cleaning and sterilizing, the iodine cup, moving animals without their water, quarantine for fish, corals and invertebrates, copper, and tracing failures.

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Key terms for this module

Read these first. Every one of them comes up in the sections below.

Biosecurity Habits that stop disease getting in or moving between systems.
Quarantine Holding a new animal in its own system until it's shown healthy.
Hospital tank A system for treating animals that are already sick.
Pathogen A virus, bacterium, parasite or fungus that causes disease.
Sterilize Kill pathogens on a cleaned tool or surface.
Povidone-iodine The iodine disinfectant used in the iodine cup.
Contact time How long a disinfectant must stay on to work.
Coral dip A bath outside the tank that removes pests from new corals.
Frag plug The base a coral fragment is glued to.

1. What biosecurity means

Biosecurity is everything done to stop disease getting into a system, or moving from one system to another.

Every system is its own island Every system is its own island System A System B System C Net ! Anything wet carries pathogens Water, nets, pens, hands, buckets and splashes

Pathogens live in the water, and you can't see them. Each system is its own island: its own water, its own animals, its own pathogens. If one system gets sick, the others stay safe as long as nothing carries water between them. Biosecurity is the habits that keep those islands apart, and that stop new animals bringing new problems in.

Clean-looking isn't clean. A net used in four systems without sterilizing links all four: if one gets sick, all four have to be watched. Every rule in this module has a reason, and knowing the reason is what gets it right when something new happens (Module 1). Personal safety around tank water is in Module 2.

If Then
It looks clean Pathogens are invisible.
One shared net Every system it touched is linked.

Check your understanding

One net was used in four systems today without sterilizing. Tomorrow one system has sick fish. Which systems could have it?

All four. The net linked them, so all four have to be watched. One skipped step turned one problem into four possible problems.

Key point Each system is an island.

2. What carries disease

Anything that carries water carries pathogens.

What carries disease Carrier How it carries Nets Wet mesh holds water and slime Buckets, cups, hoses, pumps Water inside, drops outside pH and salinity pens Dipped in one system, then the next Hands and gloves Wet skin going from tank to tank Splashes, spray Drops thrown into nearby tanks Bag water, animals Bring their own pathogens

Nets, buckets, cups, hoses, pumps and siphons hold water inside and drops outside. pH and salinity pens are dipped into one system, then the next. Wet hands and wet gloves go from tank to tank. Bubbles bursting and water splashing throw tiny drops into nearby tanks. Shipping bag water, and every animal, bring their own pathogens.

Never mix water between animals or between systems, and never move equipment between them without sterilizing it first. A UV sterilizer doesn't stop a disease spreading between animals that share the same water: once a problem is in a system, every animal in it shares it (Module 21).

If Then
Wet tool between systems Sterilize it first.
UV in a sick system Shared water still spreads it.

Check your understanding

Two systems share no water and no plumbing, and both get the same problem in the same week. What do you look for?

What moved between them: a net, a bucket, a pen, gloves, a person, an animal, splashes. If no water connects them, something carried it.

Key point Anything wet carries pathogens.

3. One system at a time

Work in an order that can't carry problems from sick to healthy.

Order of work Most vulnerable animals first Healthy systems next One system at a time ! Quarantine and sick systems last Hands washed and sprayed Before every system

Do everything for one system, log it, then move to the next. Bouncing between systems is how tools, water and hands carry problems around. Care for the most vulnerable animals first and quarantine or sick systems last, so nothing comes back from quarantine into a healthy system.

Wash hands and spray them with alcohol before switching systems, even between individual pair boxes on one rack: rub the hands together so every part, including under the nails, gets spray, then let them dry. Each system's own tools stay with it. A shared tool is sterilized before it goes into the next system.

If Then
Quarantine worked first Problems carried to healthy systems.
Hands between pair boxes Wash and alcohol spray.

Check your understanding

A coworker checks the quarantine tank first so it's done early, then does the displays. What's wrong?

Quarantine is worked last. Done first, anything on hands or tools goes from quarantine into healthy systems.

Key point Most vulnerable first, quarantine last.

4. Clean, then sterilize

Cleaning removes dirt. Sterilizing kills pathogens. Always in that order.

Clean, then sterilize Clean first Slime and dirt shield pathogens Then sterilize Dishwasher, iodine cup or alcohol Give it time A quick dip does almost nothing Rinse, dry, put away Nothing left sitting out

Slime, algae and detritus shield pathogens from a disinfectant and use it up, so scrub and rinse first. Disinfectants need contact time: a quick dip does almost nothing. Rinse afterward, because disinfectant left on a tool goes into the next system, and never pour used disinfectant into a system or a sump.

Anything that's been in the sink is washed and sterilized before it's used again, and put away after use. Buckets are washed with hot water, sprayed with alcohol and left to dry. Pumps and hoses that move salt water in or out are washed and sterilized before they go to another system. No buckets of water are left sitting around, and no equipment is left out.

If Then
Slimy tool into disinfectant Clean it first.
Used disinfectant into a sump Never.

Check your understanding

A bucket soaked in disinfectant for 10 minutes still has a ring of dried algae inside. Is it sterilized?

Not under the algae. Clean it first; then the soak counts.

Key point Clean, sterilize, rinse, put away.

5. Sterilizing methods

Choose the method by the tool: dishwasher first, the iodine cup for what can't go in it, alcohol for hands and quick surfaces.

Sterilizing methods Method Used for Dishwasher, hot cycle Anything non-electronic: the first choice Iodine cup Pens and electronic tools Alcohol spray Hands, buckets after a hot wash ! Never into a system Used disinfectant is poured away

A dishwasher's heated cycle is the first choice for anything non-electronic: nets, cups, buckets, tubing, sponges, filter socks. Fill it, run it, done. The only thing added is oxygen bleach (sodium percarbonate). Tools that can't go in a dishwasher, such as pH and salinity pens, go through the iodine cup. Alcohol spray is for hands and for surfaces after washing.

The iodine cup The iodine cup Brown = working; pale = used up 5 ml in 250 ml fresh water Graduated cup, never a guess Rinse, soak 10 minutes, rinse Last rinse in RO water ! Mix fresh every day Sooner if the color fades

The iodine cup: measure 5 ml of povidone-iodine with a graduated cup into 250 ml of fresh water. Rinse the tool in fresh water first, then soak it for at least 10 minutes with every part that touched tank water under the surface. Rinse it in RO water before it goes into another system. Iodine is brown, and the color fades as it's used up; mix a fresh cup every day, or sooner if it fades. A pH pen goes back into its storage cap after the rinse, never left in the cup.

If Then
Net or bucket Dishwasher.
pH pen Iodine cup, then RO rinse and cap.
Pale iodine cup Mix fresh.

Check your understanding

The iodine cup was mixed at 9:00 and is pale by 14:00. Five pens went through it after 13:00. What do you do?

Mix a fresh cup and put those pens through it again before they go into any system.

Key point 5 ml in 250 ml, 10 minutes.

6. Move the animal, never the water

Every animal that moves brings its water and its pathogens with it.

Move the animal, never the water Move the animal, never the water Bag or quarantine Display Animal Water ! No water moves with it Not from a bag, not from quarantine Then two rinses Destination water in two separate buckets

Shipping bag water never goes into a system, and quarantine water never goes into a display. Move the animal, not the water (Module 25 covers acclimation). No animal is moved between systems or introduced into a system without two rinses: after acclimation in its own container, it goes through water from the system it's going into, put into two separate buckets, one after the other, so as little of its old water as possible goes with it. Acclimation cups, drip lines and containers are equipment: sterilize them between animals and between shipments. Water taken out of a system for any reason never goes back into another one.

Some animals need stricter rules. Once a carpet anemone such as S. gigantea is healthy and established in a system, a new anemone never goes into that system's water, including the sump; a new carpet finishes quarantine first. Dead animals come out right away: a dead animal rots, adds waste and can spread what killed it.

If Then
Bag water Never into a system.
Moving an animal Acclimate, then two rinse buckets.
Established gigantea No new anemone in that water.

Check your understanding

A coworker pours a fish and its quarantine water straight into a display. What went wrong?

Quarantine water went into the display. Only the animal moves: acclimated in its own container, then rinsed through two buckets of display water. It can't be undone, so the display now has to be watched.

Key point Move the animal only.

7. Quarantine

Quarantine keeps a new animal in its own system until it's shown to be healthy.

A quarantine system A separate system Its own water and equipment Mature, stable water Not a bare container or bin Watched every day Behavior, breathing, fins, feeding ! Worked last After every healthy system

Quarantine holds a new arrival in a separate system, with its own water and its own equipment, so any problem it carries stays there. The quarantine system needs stable, mature water: arrivals do worst in small containers and holding bins without established filtration. Mesh containers are never used (Module 25). Quarantine is worked last, after every healthy system.

Quarantine only works if it's kept separate. Shared nets, shared pens without the iodine cup, splashes from a quarantine tank into its neighbor, or quarantine water moved with the animal all undo it. A hospital tank is different: it's for treating animals that are already sick (Module 30).

If Then
Arrival in a bare bin Mature, stable water instead.
Shared net with quarantine Quarantine undone.

Check your understanding

New fish arrive and there's no mature quarantine system ready, only an empty holding bin. What's the risk?

Arrivals do worst in containers and bins without established filtration. Quarantine needs stable, mature water.

Key point Separate, mature, watched, worked last.

8. Quarantining fish

In quarantine, the job is watching closely every day and acting fast on any sign.

Watching fish in quarantine You see What it can mean Gill flaring, rapid breathing Act now (Module 30) Any fin damage Act now (Module 30) Not eating Health warning Eats, swims, breathes normally Keep watching

Published quarantine periods for marine fish run from at least 10 days to 6 weeks or more. Watch every fish every day: how it breathes, swims, eats, and how its fins and skin look. Gill flaring, rapid breathing or any fin damage are signs to act on right away, not to wait out (Module 30 covers treatment). A fish that won't eat is a health warning.

Write down what you see each day. A log turns "it looked a bit off" into a record of when it started and what changed, which is what a treatment decision needs (Module 7).

If Then
Gill flaring Act now.
Fin damage Act now.

Check your understanding

A fish in quarantine has started flaring its gills and breathing fast. Do you wait a few days to see?

No. Those are signs to act on right away (Module 30). Log when it started.

Key point Watch and log every day.

9. Quarantining corals and invertebrates

Corals and invertebrates can carry pests that a dip and a long quarantine catch.

Quarantining corals Dip outside the tank Fresh mix for each frag Remove the plug if possible Eggs hide under the glue Inspect with a light Mornings and after dark ! 4 to 8 weeks Longer after any re-dip

New corals and invertebrates can carry parasites and predators, such as coral-eating nudibranchs, into a display. Dip each new coral outside the tank, never in a display, in a fresh mix for each frag, then rinse it in clean tank water. Remove the frag plug where possible, because pest eggs are often found where the frag is glued on. Inspect each one with a light every morning and again after the lights go out, when some pests come out.

Plan on 4 to 8 weeks of coral quarantine. Pest eggs can hide in crevices and hatch later, so re-dip whenever pests turn up and add another 4 weeks after each re-dip. Live rock is the exception: it's used for the life it brings, accepting what comes with it (Module 22).

If Then
Dip in the display Never: dip outside.
Pests found Re-dip, add 4 weeks.

Check your understanding

A new frag was dipped once and looks clean. A week later a nudibranch turns up on it. What now?

Re-dip it and add another 4 weeks of quarantine: eggs can hide in crevices and hatch later.

Key point 4 to 8 weeks, inspect with a light.

10. Copper and lasting contamination

Some contamination never washes out.

Copper stays Copper stays Held copper once ! Fish only, for good Tank, rock, sand and equipment it touched

Copper is used to treat some fish diseases (Module 30), and it's toxic to invertebrates. Any tank, rock, sand or equipment that has ever held copper is fish-only for good. Label it, keep it apart, and never let it near an invertebrate system.

Any equipment that belongs to a quarantine or treatment system stays with that system. If it has to move, it's sterilized first, and anything that held copper doesn't move to an invertebrate system at all.

If Then
Tank that held copper Fish only, for good.
Copper equipment Never near invertebrates.

Check your understanding

A spare tank was used for a copper treatment two years ago. Can it hold anemones now?

No. Anything that ever held copper is fish-only for good.

Key point Copper stays.

11. When biosecurity fails

When disease turns up, trace what moved.

When biosecurity fails You see Look for Same problem in two unconnected systems What moved between them A pale iodine cup Tools that went through it Bucket of water left sitting Where it came from and went Disease after a new arrival Bag water, skipped quarantine

The same problem in two systems that share no water: something carried it, so trace what moved between them: a net, a bucket, a pen, gloves, a person, an animal, splashes. A pale iodine cup: tools that went through it may not be sterilized; mix fresh and put them through again. A bucket of water left sitting: find where it came from and where it was going, and empty and sterilize it. Disease after a new arrival: check whether bag water went in or quarantine was skipped.

Fix the habit, not just the day. Then watch every system the carrier touched (Module 1).

Biosecurity drill: on the course website this is an interactive drill. Read what went wrong, check the tools and the work log, and fix the cause.

If Then
Two unconnected systems sick Trace what moved.
Bucket left sitting Empty and sterilize it.

Check your understanding

A bucket half full of salt water is found under a rack, and nobody knows where it came from. What do you do?

Empty it, wash and sterilize the bucket, and treat any system it may have come from or gone to as linked.

Key point Fix the habit.

12. What you see, and what it means

Same problem, two unconnected systems Something carried it.
Pale iodine cup Used up: mix fresh, redo the tools.
Bucket of water left sitting A carrier: empty and sterilize.
Quarantine worked first Order of work broken.
Bag water in a system All its pathogens go in too.
Animal moved with one rinse Two buckets of destination water.
Gill flaring in quarantine Act now.
Pest on a quarantined frag Re-dip, add 4 weeks.
Tank that once held copper Fish only, for good.

Module 23 test

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Sources

  1. WOAH Aquatic Animal Health Manual, 1.1.3 Disinfection
  2. Iowa State: Biosecurity for aquatic livestock production, facilities
  3. CFSPH Iowa State: aquaculture biosecurity checklist
  4. Natural Resources Wales: disinfecting tackle
  5. Oregon State WetVet: pathogen containment
  6. Floyd and Beleau: Aquarium fish health management (VIN)
  7. Bulk Reef Supply: Coral Rx and quarantine
  8. Michael Maddox, President of IMARCS: sterilizing methods and the dishwasher, the iodine cup, hand spraying between systems, order of work, mature water for arrivals, acclimation then two rinses in two buckets of destination water before moving or introducing an animal, the established gigantea rule, the copper rule, signs that need action