Key terms for this module
Read these first. Every one of them comes up in the sections below.
| Pathogen | An organism that causes disease: a parasite, bacterium or virus. |
| Opercula | The gill covers of a fish. |
| Quarantine | Keeping a new animal in a separate system for a period, so disease becomes apparent before it can spread. |
| Hospital container | A separate container in which an animal that's already sick is treated. |
| Marine ich | Cryptocaryon irritans, a parasite that appears as white grains on fish. |
| Trophont | The feeding stage of a parasite, on or in the fish. |
| Tomont | The cyst stage of a parasite, on surfaces, where it divides. |
| Theront | The free-swimming stage of marine ich that seeks a fish. |
| Marine velvet | Amyloodinium ocellatum, a dinoflagellate parasite of the gills and skin. |
| Copper | A former standard treatment for fish parasites, toxic to invertebrates; no longer recommended. |
| Dinoflagellate | A single-celled organism, some of which are parasites. |
| Dinospore | The free-swimming stage of marine velvet. |
| Ciliate | A single-celled organism that moves with fine hairs called cilia. |
| Brooklynella | Brooklynella hostilis, a ciliate that causes clownfish disease. |
| Uronema | Uronema marinum, a ciliate that causes red sores. |
| Formalin | A solution of formaldehyde used to treat some parasites; toxic and carcinogenic. |
| UV sterilizer | A lamp that kills organisms in the water flowing past it (Module 21). |
| Micron | One thousandth of a millimeter; filter socks are rated by the particle size they catch. |
| Freshwater dip | A brief bath in fresh water matched to the animal's temperature and pH. |
| Ciprofloxacin | An antibiotic used to treat bacterial infections in fish and anemones. |
| Stock solution | A measured amount of a drug dissolved in water, dosed from in mL. |
| Ammonia neutralizer | A product that binds ammonia so that it isn't toxic to fish. |
| Nitrifying bacteria | Bacteria that convert ammonia to less toxic compounds; the biofilter. |
| Perkinsus | A protozoan parasite of clams with no known treatment. |
| Byssal tissue | The tissue that produces a clam's anchoring threads. |
| Tissue necrosis | Death and loss of coral tissue from the skeleton (RTN when rapid, STN when slow). |
| Brown jelly disease | A brown, jelly-like mass that consumes coral tissue, most often in large-polyp corals. |
| Black band disease | A dark band of microorganisms that moves across a coral, leaving bare skeleton. |
| Euthanize | To end an animal's life humanely. |
1. What healthy looks like
Recognizing disease depends on knowing each animal's normal appearance and behavior, and checking it every day.
A healthy fish rises and feeds as soon as food is added, breathes evenly with its gill covers (opercula) closed between breaths, holds its fins open, and has clear skin with no spots, film or sores. Its behavior is normal for its species: some clownfish rest on the bottom, and longfin forms lie on their side, without being ill (Module 29).
A healthy invertebrate is expanded, colored and attached as is normal for its species. Anemones, clams and corals are covered in Modules 27 to 29.
Observe every animal at least once a day, ideally at feeding, when appetite and breathing are easiest to judge. Record anything unusual with the date and time. A log turns an impression into a record of when a problem began and what changed, which is what a treatment decision requires (Module 7).
| If | Then |
|---|---|
| Feeding | Rises and eats at once. |
| Breathing | Even, gill covers closed between breaths. |
| Check | Daily, at feeding; log anything unusual. |
Check your understanding
A fish hides at feeding time for the first time in months. Is that worth recording?
Yes. A change from its normal behavior is the earliest sign of a problem. Record it, check the water, and watch that fish closely.
Key point Know normal first.
2. Why animals become sick
Most outbreaks need both a pathogen and a weakened animal. Stress is usually what tips the balance.
A pathogen is an organism that causes disease: a parasite, bacterium or virus. Many pathogens are present at low levels in an established system without causing harm. An outbreak follows when animals are weakened by stress: shipping and handling, aggression, injury, crowding, or poor or unstable water.
New arrivals are the most common source of new pathogens, and the most stressed animals in a system. This is why every new animal is quarantined, kept in a separate system for a period so that any disease becomes apparent before it can spread (Module 23), and why captive-bred animals, which carry fewer pathogens, are preferred (Module 24).
Prevention is therefore mostly husbandry: stable water, appropriate stocking, correct feeding, quarantine and biosecurity. Treatment is needed when prevention has failed.
| If | Then |
|---|---|
| Pathogen | Often already present. |
| Stress | Shipping, aggression, injury, poor water. |
| New arrivals | Quarantine first (Module 23). |
Check your understanding
A system that has been stable for a year suffers an ich outbreak a week after a new fish is added without quarantine. What most likely happened?
The new fish brought the parasite, or the stress of the addition let one already present take hold. Quarantine would have contained it.
Key point Prevention is husbandry.
3. Reading the signs
Signs point toward a cause, but several diseases look alike, and poor water can mimic all of them.
Check the water first. Rapid breathing, hiding and refusing food are also signs of ammonia, low dissolved oxygen, a temperature swing or a salinity error. Test before you diagnose, and correct the water whatever the diagnosis.
Then read the animal. Note which signs are present, how quickly they appeared, and how many fish, and which species, are affected. White grains like salt point to marine ich. A fine gold, dusty sheen with very rapid breathing points to marine velvet. Thick white mucus points to Brooklynella, especially in clownfish. Red sores with defined edges point to Uronema. Flared gill covers and fin damage point to a bacterial infection.
Signs overlap, and an animal can have more than one disease at once. When the signs don't fit one cause clearly, isolate the animal and treat for the most dangerous likely cause first: velvet and Brooklynella can kill within one to two days, while ich usually progresses more slowly.
| If | Then |
|---|---|
| First | Test the water. |
| White grains | Marine ich. |
| Gold dust, very fast breathing | Velvet: an emergency. |
Check your understanding
Every fish in a system is breathing rapidly at the surface, with no spots or sores. What do you check first?
The water, dissolved oxygen above all. When every species shows the same sign at once, the water is the likeliest cause.
Key point Treat the most dangerous likely cause first.
4. Marine ich
Marine ich is the most common parasite of marine fish. Only its free-swimming stage can be attacked.
Marine ich, Cryptocaryon irritans, appears as white grains like grains of salt on the body and fins. Its life cycle has three stages. The trophont feeds within the skin, protected from treatment. It then drops off and becomes a tomont, which forms a cyst on rock, sand or glass and divides into many new parasites, also protected. These leave as theronts, the free-swimming stage that seeks a new fish. Only the theronts are vulnerable, so treatment must outlast the whole cycle, and a course commonly runs four to six weeks (Module 15).
Control. Ich is controlled with a correctly sized UV sterilizer, which kills theronts carried past the lamp (Module 21). In a bad outbreak, combine it with 5 or 10 micron filter socks to catch free-swimming stages, and increase water movement so that more of them are carried to the UV and the socks. With properly sized UV, ich can usually be controlled, and often eventually eliminated. This works in reef systems, where medication can't be used, as well as in fish-only systems.
Copper. Copper was once the standard treatment for ich and velvet. It's no longer recommended: UV and fine mechanical filtration do the job. Copper is toxic to invertebrates, and any tank, rock, sand or equipment that has ever held it's fish-only for good (Module 23).
| If | Then |
|---|---|
| Control | UV, 5 or 10 micron socks, more flow. |
| Copper | No longer recommended. |
| Course | Four to six weeks. |
Check your understanding
A reef system with corals and clams has an ich outbreak. A coworker proposes copper. What do you say?
Copper is no longer recommended, and in a reef it would kill the invertebrates and leave the system fish-only for good. Use correctly sized UV, 5 or 10 micron socks and extra water movement.
Key point Only the theront can be attacked.
5. Marine velvet
Marine velvet is faster and deadlier than ich. Treat it as an emergency.
Marine velvet, Amyloodinium ocellatum, is a parasitic dinoflagellate that attacks the gills and skin. The first sign is often a dead or dying fish. Affected fish stop feeding, breathe very rapidly and may show a fine, gold or dusty sheen on the skin, finer than the grains of ich. Its life cycle resembles that of ich, with a protected cyst stage and a much smaller free-swimming stage, the dinospore.
Isolate at once. Move affected fish to a hospital container of mature system water with strong aeration (section 8). A freshwater dip, with the water matched to the fish's temperature and pH, removes many of the parasites on the fish, but a single dip isn't a cure, because the cyst stage is protected.
Control. In the display and the hospital container, use 5 micron filter socks, which catch the dinospores that pass a 10 micron sock (Module 15), run correctly sized UV, which reduces their numbers, and keep water moving so they reach both. Add no new fish until the outbreak has ended. Velvet moves in days, not weeks: delay is how fish are lost.
| If | Then |
|---|---|
| Speed | Days, not weeks. |
| Fish | Isolate at once; strong aeration. |
| Control | 5 micron socks, UV, more flow. |
Check your understanding
A fish breathing very rapidly has a fine gold dust on its body. A coworker wants to watch it until tomorrow. What's the risk?
Velvet can kill within days, often sooner. Isolate it in a hospital container with strong aeration now, and run 5 micron socks and UV.
Key point Act at once.
6. Brooklynella and Uronema
These ciliates live and multiply directly on the fish and spread quickly.
Brooklynella hostilis attacks the gills and skin and is known as clownfish disease, because it's especially common in wild-caught clownfish. Early signs are folded fins and lethargy, followed by a thick white mucus coat and rapid breathing. It can kill a fish within 24 to 48 hours of the first signs. Antibiotics don't treat it; formalin is the most effective treatment, used strictly as directed on the product. Captive-bred clownfish carry it far less often.
Uronema marinum causes red sores, often with a sharply defined edge, and is especially common in chromis and other fish stressed by shipping. It progresses quickly, and fish with open sores have a poor outlook. Isolate affected fish at once.
Formalin safety. Formalin is a solution of formaldehyde: toxic, irritating and a known carcinogen. Wear gloves and eye protection, work in a ventilated area, and use it only in a separate treatment container, never in a display system. It lowers dissolved oxygen, so aerate heavily during treatment.
| If | Then |
|---|---|
| Brooklynella | Thick white mucus; formalin. |
| Uronema | Red sores; isolate at once. |
| Formalin | Gloves, eye protection, ventilation. |
Check your understanding
A newly imported wild clownfish has a thick white coat of mucus and is breathing fast. A coworker starts an antibiotic. What's wrong?
That points to Brooklynella, which antibiotics don't treat. It needs formalin, in a treatment container, at once.
Key point Antibiotics don't treat them.
7. Bacterial infections
Bacterial infections are common in stressed and newly shipped fish. Caught early, they respond quickly to antibiotics.
Signs. The earliest signs are flared gill covers and fast or labored breathing. Fin damage follows, even small: white, cloudy or frayed fin edges, strands hanging from the fins, fins shorter than a few days before, or a white haze on the body. A fish lying on its side and refusing food is in the late stage, when treatment rarely saves it. Don't wait to see whether it worsens.
Before treating, check that the signs aren't a white mucus coat on the skin and gills. That points to Brooklynella, which antibiotics don't treat.
Ciprofloxacin. Fish are treated in a hospital container at 6.5 mg/L. One 500 mg tablet dissolved whole in 500 mL of RO water gives a stock of 1 mg per mL, so 6.5 mL of stock per liter gives 6.5 mg/L. Keep the container dark or covered, because light degrades the drug. Photograph the fins daily and compare them with the previous day.
Duration. Treat for at least five days, even if the fish looks normal sooner. If it's improving slowly, continue for up to ten days. If there's no improvement at day five, the medication is changed.
| If | Then |
|---|---|
| Earliest sign | Flared gill covers, fast breathing. |
| Ciprofloxacin | 6.5 mg/L in a hospital container. |
| No improvement at day five | Change the medication. |
Check your understanding
A fish on Ciprofloxacin looks normal on day three. Can treatment stop?
No. The course runs at least five days, even when the fish looks normal sooner.
Key point At least five days; up to ten.
8. The hospital container
A sick fish is treated in its own container, where medication can be controlled and nothing reaches the display.
A hospital container holds an animal that's already sick, unlike quarantine, which holds new arrivals (Module 23). Fill it with mature system water, add aeration and a heater, and keep it at the system's temperature. Never use a mesh container: fine mesh clogs with biofilm, oxygen falls, and the fish suffocates (Module 25).
Ammonia. Medications such as antibiotics kill nitrifying bacteria, so a treatment container has no working biofilter. Add an ammonia neutralizer, test ammonia daily, and change water with system water whenever ammonia rises, dosing the medication only for the new water added.
One medication at a time. Don't add a second medication on top of the first. Don't give methylene blue to a fish that's already struggling to breathe.
Biosecurity. Every tool that enters a treatment container is sterilized before it goes in and again when it comes out, and no water passes between the container and any system (Module 23).
| If | Then |
|---|---|
| Water | Mature system water, aeration, heater. |
| Ammonia | Neutralizer; test daily. |
| Medications | One at a time. |
Check your understanding
A fish in a hospital container is on Ciprofloxacin, and ammonia has risen. What do you do?
Change water with system water and dose Ciprofloxacin only for the new water added; keep the ammonia neutralizer in and test daily.
Key point No mesh; nothing crosses.
9. Invertebrate diseases
Anemones, clams and corals have their own diseases, and most can't be treated with the medications used for fish.
Anemones. A limp anemone with a slack or gaping mouth, often with cloudy water after lights out, has a bacterial infection, treated with Ciprofloxacin at a dose that depends on the species (Module 29).
Giant clams. A pinched mantle has several possible causes, of which pinched mantle disease is treated with a freshwater dip (Module 28). Perkinsus, a protozoan parasite of clams, causes a mantle that doesn't extend fully, slow responses to shadow and touch, and sloughing of the byssal tissue in the days before death. There's no treatment. Clams that show these signs don't recover, so symptomatic clams are euthanized with ethanol rather than left to decline in the system. Perkinsus spreads through water and equipment, so strict biosecurity is the only protection (Module 23).
Corals. Tissue necrosis, the loss of tissue from the skeleton, is called rapid tissue necrosis (RTN) when it moves quickly and slow tissue necrosis (STN) when it moves slowly; its cause isn't fully understood, and it's often linked to a stress event such as a temperature spike. Tissue loss isn't always disease. Allelopathy (Module 24), nutrients bottoming out at zero nitrate or phosphate (Module 17), a parameter swing, handling, stings from neighbors and pests (Module 31) all cause it, so diagnose before treating. A failing colony can sometimes be saved by cutting it at least 1.3 cm ahead of the dying tissue.
Brown jelly disease covers large-polyp corals with a brown, jelly-like mass. Remove the jelly with a turkey baster, then treat the coral with Ciprofloxacin at 1 mg/L in a treatment container. When several corals in a display are affected and can't be removed, the low in-system dose used for anemones can be applied to the whole display: 0.125 mg/L every 12 hours for 7 to 14 days, watching every animal throughout (Module 29). Black band disease is a dark band, a mat of microorganisms, that moves across a colony and leaves bare skeleton behind.
| If | Then |
|---|---|
| Anemone infection | Ciprofloxacin (Module 29). |
| Perkinsus in clams | No treatment; euthanize with ethanol. |
| Coral tissue loss | Diagnose first; then cut 1.3 cm ahead. |
| Brown jelly | Baste it off; Ciprofloxacin 1 mg/L. |
Check your understanding
A clam's mantle hasn't extended fully for several days, it reacts slowly to shadow, and its byssal tissue is sloughing. What's the likely cause, and what's done?
Perkinsus. There's no treatment, so the clam is euthanized with ethanol, and strict biosecurity protects the rest.
Key point Fish medications aren't for invertebrates.
10. When animals get sick
Disease and poor water often occur together. Check the water, read every animal, isolate and treat early, and correct each cause.
Begin with the water: ammonia, dissolved oxygen, temperature and salinity. A water problem can cause every sign in this module, and it weakens animals so that pathogens take hold. Correct it whatever else is found.
Then read every animal. One sick fish suggests an individual problem; several species at once suggests the water or a contagious parasite. Note how quickly signs appeared: velvet and Brooklynella move in one or two days, ich and most bacterial infections over several.
Isolate and treat early, with the treatment that fits the cause. Then correct what allowed the outbreak: a new arrival that skipped quarantine, a stressful move, aggression or unstable water. Most outbreaks have more than one cause.
Fish health drill: on the course website this is an interactive drill. Read the signs, check each part, and correct every cause.
| If | Then |
|---|---|
| First | The water. |
| Several species sick | Water or a contagious parasite. |
| Afterward | Correct what allowed it. |
Check your understanding
Three species in one system begin breathing rapidly on the same morning, and ammonia reads high. What comes first?
The water: correct the ammonia at once. Then watch each fish for signs that remain once the water is right.
Key point Most outbreaks have more than one cause.
11. Signs and responses
| White grains like salt on body and fins | Marine ich: correctly sized UV, 5 or 10 micron socks, more flow. |
| Gold, dusty sheen, very rapid breathing | Marine velvet: isolate now; 5 micron socks, UV. |
| Thick white mucus, rapid breathing, clownfish | Brooklynella: formalin; antibiotics don't treat it. |
| Red sores with defined edges | Uronema: isolate at once. |
| Flared gill covers, fin damage | Bacterial infection: Ciprofloxacin 6.5 mg/L. |
| Lying on its side, refusing food | Late stage; act on earlier signs next time. |
| Every species breathing fast at once | Check the water first. |
| Ammonia rising in a treatment container | Water change; dose only the new water. |
| Clam mantle not extending, byssus sloughing | Perkinsus: no treatment; euthanize with ethanol. |
| Coral tissue peeling from the skeleton | Diagnose first: allelopathy, zero nutrients, a swing or disease. |
| Brown jelly over coral polyps | Baste the jelly off; Ciprofloxacin 1 mg/L in a container. |
| Several corals affected, can't be removed | Low in-system dose: 0.125 mg/L every 12 hours. |
Module 30 test
30 questions. Pass with 27 or more. Opens after you pass every section check in this module. Sign in to start.
Sources
- University of Florida IFAS: Cryptocaryon irritans infections in marine fish, FA164
- University of Florida IFAS (Reed and Francis-Floyd): Amyloodinium infections of marine fish, VM90
- Virginia Tech (Smith and Schwarz): Getting acquainted with Amyloodinium ocellatum
- University of Florida IFAS (Yanong): Use of copper in marine aquaculture and aquarium systems, FA165 (copper's long use against marine parasites)
- Zuku Review: top infectious aquatic diseases (only theronts are susceptible; Cryptocaryon treatment 4 to 6 weeks)
- Reef Builders: Brooklynellosis, a subtle, fast-moving killer
- VIN: A review of Brooklynella parasitization in marine fishes
- Bulk Reef Supply: quarantine phase 2, Brooklynella and Uronema (formalin)
- Reef Central: Brooklynella hostilis and Uronema marinum (lesion margins; formalin hazards)
- Sheppard and Phillips (2008): Perkinsus olseni in reef clams Tridacna crocea. Dis Aquat Org 79:229-235
- Canadian Food Inspection Agency: Perkinsus olseni (spread by water and equipment; no treatment)
- Bulk Reef Supply: coral tissue necrosis, the mystery of RTN and STN
- Sweet and Bythell: Coral diseases in aquaria and in nature. J Mar Biol Assoc UK (2012)
- ReefAquarium.com: coral diseases (black band disease)
- Reef Stable: why is my coral dying (RTN, STN, brown jelly disease)
- Manta Systems: Uronema (rapid progression, red sores)
- Manta Systems: Brooklynella (treatments lower oxygen; aerate)
- Michael Maddox, President of IMARCS: husbandry practice and observations
